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Image Search Results
Journal: bioRxiv
Article Title: Alternative polyadenylation drives isoform-dependent m⁶A remodeling during Zika virus infection
doi: 10.64898/2026.03.27.714597
Figure Lengend Snippet: A) Experimental workflow of GLORI-seq protocol to identify differential m 6 A following ZIKV infection (MOI 1, 48 hpi, n=3). B) Scatter plot showing ZIKV-induced changes in m 6 A levels of individual sites. Orange and blue dots represent upregulated (n=2004) and downregulated (n=502) m 6 A sites during ZIKV infection, respectively. C) Representative gene track plots of upregulated m 6 A in RIOK3 (upper panel), downregulated m 6 A in CIRBP (middle panel), and unchanged m 6 A in ACTB (lower panel). D) Metagene profiles showing the distributions of upregulated, downregulated, and unchanged m 6 A sites across the mRNA molecule. E) The top 6 motifs across upregulated, downregulated, and unchanged m 6 A sites and the proportion of m 6 A sites they contain. F) The absolute difference in m 6 A level of upregulated and downregulated m 6 A sites across the top 6 motifs. G) PANTHER GO enrichment analysis for genes with upregulated m 6 A sites using a binomial test with FDR correction (FDR p < 0.05).
Article Snippet: 2.5 μg of HCV PAMP RNA was transfected into cells for 8 h using the
Techniques: Infection
Journal: bioRxiv
Article Title: Alternative polyadenylation drives isoform-dependent m⁶A remodeling during Zika virus infection
doi: 10.64898/2026.03.27.714597
Figure Lengend Snippet: A) and B) The correlation of m 6 A levels for sites detected by GLORI-seq between three biologically independent samples of mock- (A) or ZIKV-infected (B) Huh7 cells (48 hpi, MOI 1). C) Boxplots showing distribution of methylation level of m 6 A sites in Mock and ZIKV conditions. D) Principal component analysis of GLORI-seq m 6 A stoichiometry rates for Mock and ZIKV conditions. E) Metagene profiles showing the distributions of Mock and ZIKV m 6 A sites across the mRNA molecule. F) The top 6 motifs across Mock and ZIKV m 6 A sites and the proportion of m 6 A sites they contain.
Article Snippet: 2.5 μg of HCV PAMP RNA was transfected into cells for 8 h using the
Techniques: Infection, Methylation
Journal: bioRxiv
Article Title: Alternative polyadenylation drives isoform-dependent m⁶A remodeling during Zika virus infection
doi: 10.64898/2026.03.27.714597
Figure Lengend Snippet: A) and B) RT-qPCR analysis (relative to RPL30 ) of RNA expression of ALCAM-S and ALCAM-L (A) and NHSL1-S and NHSL1-L (B) upon DENV infection (48 hpi, MOI 1). C) and D) RT-qPCR analysis (relative to RPL30 ) of RNA expression of ALCAM-S and ALCAM-L (C) and NHSL1-S and NHSL1-L (D) upon WNV infection (48 hpi, MOI 0.01). E) and F) RT-qPCR analysis (relative to RPL30) of RNA expression of ALCAM-S and ALCAM-L (E) and NHSL1-S and NHSL1-L (F) upon HCV infection (48 hpi, MOI 1). G) and H) RT-qPCR analysis (relative to RPL30 ) of RNA expression of ALCAM-S and ALCAM-L (G) and NHSL1-S and NHSL1-L (H) upon transfection with HCV PAMP (8 hrs).
Article Snippet: 2.5 μg of HCV PAMP RNA was transfected into cells for 8 h using the
Techniques: Quantitative RT-PCR, RNA Expression, Infection, Transfection